Cell Culture

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چکیده

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Mesenchymal Stem Cell Purification from the Articular Cartilage Cell Culture

Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Artic...

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Cell Culture

Summer time is vacation time—a chance to step away from minipreps or grant deadlines and investigate nature outside the lab. For many of us, that means a trip to the beach. But don't let those colorful umbrellas and vigilant lifeguards fool you. Every foray into nature is ultimately a battle between predator and prey. In this Cell Culture, we explore cellular and molecular mechanisms used by pr...

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Cell Culture

On June 11 th , the best soccer players from around the world will meet in South Africa at the FIFA 2010 World Cup. To reach this level of competition, these players combine phenomenal ball-handling skills with three major characteristics: fast sprinting, rapid reaction times, and ferocious tenacity. This issue's Cell Culture explores these three athletic qualities from a molecular and cellular...

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Mouse liver cell culture. II. Primary culture.

Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (less than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater that 2 h resulted i...

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Isolation and detection of Mycoplasma pneumoniae from cell culture by culture and PCR.

Background: Mycoplasma pneumoniae is one of the major species of mycoplasmas which could contaminate cell cultures. Identification of M. pneuminae is significant because Mycoplasmas could contaminate and have unsuitable effect on the cell cultures. Rapid detection of these contaminations is so important and it could be significant role in preventing and controlling of contamination in cell cul...

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ژورنال

عنوان ژورنال: Equilibrium Research

سال: 2008

ISSN: 0385-5716,1882-577X

DOI: 10.3757/jser.67.1